Composite

Part:BBa_K2970008:Design

Designed by: Alexander Bast   Group: iGEM19_Hamburg   (2019-10-03)


Gate/Trigger test composition


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 103
    Illegal NheI site found at 284
    Illegal NheI site found at 422
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1219


Design Notes

Secondary structures make it hard to assemble this part.

Source

References

Green, A.A. et al. "Complex cellular logic computation using ribocomputing devices." (Nature, 2017)